Detection of the biotin–streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes

0167-9317/$ see front matter 2008 Elsevier B.V. A doi:10.1016/j.mee.2008.11.058 * Corresponding author. Address: Institute of Micro Terma Patriarxou Grigoriou Street, Agia Paraskevi 153 E-mail addresses: [email protected], vtsou The detection of the biotin–streptavidin interaction is presented based on surface stress changes of functionalized Si membranes. During the biomolecular interaction, the surface stress of the functionalized membranes alters resulting in their deflection and finally in capacitance variations. The biological interaction on the sensors surface was verified after each experiment using fluorescent microscopy. Successful detection of 21 nM of streptavidin using 3.55 mM biotin probes is presented. 2008 Elsevier B.V. All rights reserved.